Semenya will take to the track for her two-lap final at 8.10pm tonight with a two-year unbeaten record to defend.
She is the reigning Olympic champion and has won gold (2009) and silver (2011) in the world final before.
The South African already has a medal from these championships after snatching bronze in the 1500m and is looking to win back her world title.
However, the 26-year-old is a near-constant source of controversy due to her hyperandrogenism, a condition that gives her naturally higher levels of testosterone.
Caster Semenya is hoping to run past all the controversy and claim gold
World Athletics Championship 2017 prize money revealed
Tue, August 1, 2017
Click through the gallery to reveal prize money totals for the World Athletics Championship 2017
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8th: $4,000 (Individual) $4,000 (Relay)
She has previously been forced by the IAAF to take hormone-reducing drugs to be allowed to compete before the Court of Arbitration for Sport (CAS) overturned the ruling.
The debate rages on with British athlete Lynsey Sharp calling for the authoritities to “sort it out”.
But Semenya appears unflustered by the speculation that follows her almost everywhere she goes.
"I've no time for nonsense. Medication or no medication,” Semenya said.
Lynsey Sharp is one of those who have criticised authorities for allowing Caster Semenya to race
"For me, it's their own decisions.
"I really don't have time for nonsense.
"Those are the things, the issues, that I don't focus on.
“It's not my business. It's their business.
"Those are the writings that I've being seeing since 2009.
"Sometimes, you get annoyed or you get bored.
"For me, it's the past. Whoever deals with it, it's their business, not mine.
"I'm not even bothered.
"I'm the kind of person who doesn't really focus on more negativity.
“I’m a positive person and I look at things in a positive way.”